We narrowed to 4,720 results for: donor
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Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-mEGFP
Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-mEGFP
Plasmid#115910Purposedonor to insert EGFP at the SEC61B locus in human cellsDepositorAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-dTomato
Plasmid#115919Purposedonor to insert IRES-dTomato at the GAPDH locus in human cellsDepositorInsertIRES-dTomato
UseDonor templateTagsdTomatoAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP1723
Plasmid#222727PurposesfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)DepositorTypeEmpty backboneTagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-N57
Plasmid#169920PurposeExpression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-N57
UseCRISPRMutationN57 is the PAI domain of SB100XPromoterCAGAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-SB
Plasmid#169919PurposeExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-SB100X
UseCRISPRPromoterCAGAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.DepositorInsertattP
UseBxb1ExpressionMammalianAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kusabira Orange-C1 (KO)
Plasmid#54792PurposeLocalization: C1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneTagsKusabira OrangeExpressionMammalianAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway CloningAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage sitePromoterpolyhedrinAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GG
Plasmid#213039PurposeAAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)DepositorTypeEmpty backboneUseAAV; Vector for golden gate assemblyTagsN/APromoterN/AAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-dTomato
Plasmid#115920Purposedonor to insert dTomato at the SEC61B locus in human cellsDepositorInsertSEC61B-dTomato
UseDonor templateTagsdTomatoAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-mEGFP
Plasmid#115911Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-iTagRFP-T
Plasmid#115916Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T
UseDonor templateTagsTagRFP-TAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only