We narrowed to 5,554 results for: donor

1. Epac-S-H96

   Plasmid

   #170342

   Purpose

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1788

   Plasmid

   #154317

   Purpose

   CT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1787

   Plasmid

   #154316

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-actin-G168D,Y169D

   Plasmid

   #118384

   Purpose

   Gateway compatible donor vector with actin-G168D,Y169D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   G168D,Y169D

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Solo-mStayGold-LMNB1

   Plasmid

   #227328

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mScarlet-MAPRE1

   Plasmid

   #227323

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-KO-Puro-TP53

   Plasmid

   #227319

   Purpose

   Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-EZR

   Plasmid

   #227294

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   EZR Homology Arms flanking a mStayGold Tag (EZR Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mStayGold-EFS-Blast-ARL13B

   Plasmid

   #227278

   Purpose

   Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-Solo-mStayGold-H3C2

    Plasmid

    #227334

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid

    #227335

    Purpose

    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid

    #227333

    Purpose

    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Solo-mStayGold-MAP4

    Plasmid

    #227296

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-Solo-mStayGold-CEP192

    Plasmid

    #227289

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-Solo-mStayGold-ARL13B

    Plasmid

    #227277

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-mStayGold-EFS-Puro-CEP192

    Plasmid

    #227290

    Purpose

    Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Puro XLone-CCND2

    Plasmid

    #179845

    Purpose

    donor plasmid for inducible expression of CCND2 in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR, Luciferase, and TALEN ; Donor plasmid

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. B2M-SDMutation-EhT

    Plasmid

    #215546

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    

    Use

    CRISPR

    Mutation

    The second base of the first intron of B2M (From …

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. B2M-SDMutation-EPG

    Plasmid

    #215545

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    

    Use

    CRISPR

    Mutation

    The second base of the first intron of B2M (From …

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid

    #207772

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Feb. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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