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pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site D (refer to cita…

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site C (refer to cita…

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site B (refer to cita…

Promoter

Available since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site A (refer to cita…

Promoter

Available since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

Plasmid

#119945

Purpose

Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Renilla luciferase

Use

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash5

Plasmid

#74133

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Akaluc (neo)

Plasmid

#124701

Purpose

Aka-Luciferase reporter

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Akaluciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hPGK

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti HRE mutant-Luc pGK Hygro

Plasmid

#118707

Purpose

reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HREmutant-Luc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

Plasmid

#164615

Purpose

Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

Depositor

Jeffrey Chao

Article

Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

Insert

Renilla luciferase

Use

Tags

24xMS2v5 (3'UTR) and SunTag

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash6

Plasmid

#74134

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
Rlucrarr3-Flash1

Plasmid

#74129

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
Rlucrarr3-Flash4

Plasmid

#74132

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
Rlucrarr3-Flash3

Plasmid

#74131

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
Rlucrarr3-Flash2

Plasmid

#74130

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
Gal4-TET1-878-911-WT(pL84)

Plasmid

#124400

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911 wildtype (TET1 Human)

Use

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSI-check2-CUL1-3'UTR

Plasmid

#97156

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

CUL1 3'UTR (CUL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-STAT1 5'-UTR

Plasmid

#115353

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of STAT1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

STAT1 5'-UTR (STAT1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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phFluc-WT

Plasmid

#177714

Purpose

Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

Depositor

Erik Böttger

Article

Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

Insert

hFluc WT

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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