We narrowed to 2,598 results for: pCas

Showing: 1321 - 1340 of 2598 results

Results per page:

Sort results by:

ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
px459V2.0_Conc2

Plasmid

#134451

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Publication

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pMJ923

Plasmid

#78313

Purpose

Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

Depositor

Martin Jinek

Publication

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-mCherry-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCMV-dCas13-inactive M3M14nes

Plasmid

#157855

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
xABEmax

Plasmid

#119813

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pX330-BsaIx2-DD-Cas9

Plasmid

#75380

Purpose

Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

Depositor

Michele Calos

Publication

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Publication

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1028-ABE8e

Plasmid

#138492

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (CP1028)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Expression

Mammalian

Mutation

VQR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
SpyCas9 PE2*

Plasmid

#169850

Purpose

Mammalian Expression, SpyCas9 prime editor, optimized NLS

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

spCas9 PE*

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Publication

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Publication

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pRDA_426

Plasmid

#179097

Purpose

BE Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e

Use

CRISPR and Lentiviral

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CANE-LV envelope

Plasmid

#86666

Purpose

pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

Depositor

Fan Wang

Publication

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

CANE-LV envelope

Use

Lentiviral, Mouse Targeting, and Synthetic Biolog…

Expression

Mammalian

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles