We narrowed to 5,328 results for: pcdna3.1

1. pcDNA3.1/Myc-His(-)-HSulf-1

   Plasmid

   #13003

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 1 (SULF1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/V5-His Snk/Plk2

   Plasmid

   #16015

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-DRD2-cpHalo∆-mEGFP

   Plasmid

   #194679

   Purpose

   CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   DRD2_cpHalo∆
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(Hygro) TagBFP-hNucleolin

   Plasmid

   #182592

   Purpose

   expresses bfp-hNucleolin in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hNucleolin (NCL Human)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag-HIF1B (#930)

   Plasmid

   #99916

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ArchT-BFP2-TSERex

   Plasmid

   #123312

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   ArchT-BFP2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

   Plasmid

   #138983

   Purpose

   IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

    Plasmid

    #138990

    Purpose

    IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

    Plasmid

    #138989

    Purpose

    IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

    Plasmid

    #138988

    Purpose

    IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

    Plasmid

    #138987

    Purpose

    IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

    Plasmid

    #138986

    Purpose

    IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

    Plasmid

    #138985

    Purpose

    IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

    Plasmid

    #138984

    Purpose

    IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

    Plasmid

    #117484

    Purpose

    Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyCatcher002-6aa-sfCherry2(1-10)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid

    #89928

    Purpose

    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    rat Insulin II promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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