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pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

Plasmid

#113158

Purpose

An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

mU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRCaMP1

Plasmid

#125245

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP1

Expression

Mammalian

Mutation

W44Y

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript alpha-SSeCKS

Plasmid

#61295

Purpose

contains rat alpha SSeCKS cDNA

Depositor

Irwin Gelman

Insert

alpha SSeCKS (Akap12 Rat)

Expression

Bacterial

Mutation

cDNA with 1kb of 3' UTR and 150bp of 5'…

Promoter

T7

Available Since

Dec. 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T Doc2beta C2AB(125) G361C

Plasmid

#134694

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

G361C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PS-CFP2-Homer1-N-18

Plasmid

#57229

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pOC1 Gria1-HA KI

Plasmid

#183427

Purpose

CreON knock-in for GluA1-HA (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo KI

Plasmid

#183449

Purpose

CreOFF knock-in for PSD95-Halo (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-gamma2s

Plasmid

#118036

Purpose

GABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRG2 (Gabrg2 Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-mCitrine

Plasmid

#61676

Purpose

Single mCitrine-labeled, truncated kinesin-1

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KHC(1-560)-mCitrine (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-CathespinB-6

Plasmid

#54024

Purpose

Localization: Lysosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

CathespinB

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

Plasmid

#107736

Purpose

In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

Depositor

Rylan Larsen

Insert

synaptophysin-GCaMP6s, mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

nEF

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEF6 rglut1 HA complete

Plasmid

#89572

Purpose

rglut1 HA complete

Depositor

Jeffrey Rathmell

Article

Wieman et al Mol Biol Cell. 2007 Apr;18(4):1437-46. E

Insert

pEF6 rglut1 HA complete (Slc2a1 Rat)

Tags

V5-His A, B, C and rglut1 HA complete

Expression

Mammalian

Promoter

EF-1α

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22, G120A)

Plasmid

#45449

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120, Glycine at position 12…

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-GFP11

Plasmid

#61670

Purpose

Non-fluorescent, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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RnAT1aR-mCherry

Plasmid

#137782

Purpose

Visualization of At1aR

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat AT1a receptor (Agtr1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE0

Plasmid

#107678

Purpose

Expresses dLbCpf1-BE0 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0

Expression

Mammalian

Mutation

D832A/E925/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pOC2 CRISPIE-Halo-Homer1 KI

Plasmid

#183446

Purpose

CreON knock-in for Halo-Homer1 (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and CRISPIE Halo donor

Use

CRISPR

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA GAL4 CREB 1-341

Plasmid

#22972

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only