We narrowed to 2,970 results for: PPL

1. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

   Plasmid

   #224490

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Golgi-mTagBFP-P2A-H2A-iRFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9-NAT

   Plasmid

   #64329

   Purpose

   Cas9 expression cassette carried by a yeast single-copy episome plasmid

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1_oROS-HT

   Plasmid

   #216413

   Purpose

   Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME Actin Vhh mCherry (JDW 1240)

   Plasmid

   #224499

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mCherry
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

   Plasmid

   #35510

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. FASTHDR-Cterm-mClover3-Puromycin

   Plasmid

   #167205

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV SYN flex PSAM4 GlyR IRES EGFP

   Plasmid

   #119741

   Purpose

   Cre-dependent chemogenetic inhibitor expression

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5 and AAV9

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.17-m

   Plasmid

   #136298

   Purpose

   expresses ABE8.17-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.17-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8.13-m

    Plasmid

    #136296

    Purpose

    expresses ABE8.13-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGM202T7

    Plasmid

    #69993

    Purpose

    E. coli/Streptomyces expression vector containing the T7-promoter

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    

    Use

    Tags

    N-terminal and C-terminal His tag encoding sequen…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FASTHDR-Cterm-mRuby3-Zeo

    Plasmid

    #167206

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FASTHDR-Cterm-mTagBFP2-Blasticidin

    Plasmid

    #167207

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231365

    Purpose

    minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231366

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

    Plasmid

    #231370

    Purpose

    SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. p5E-dr-myl7-pro (JDW 1148)

    Plasmid

    #229836

    Purpose

    A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    cmlc2/myl7
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ABE8.20-d

    Plasmid

    #136301

    Purpose

    expresses ABE8.20-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmIL-6promoterEGFP

    Plasmid

    #112896

    Purpose

    To monitor the activation of the murine IL-6 promoter by eGFP expression

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA1

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-ArchT-GFP

    Plasmid

    #29777

    Purpose

    AAV-mediated expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OptoProfilin

    Plasmid

    #208286

    Purpose

    Expresses Cry2(1-531)-mCherry-Profilin.WT

    Depositor

    Robert Hughes

    Article

    Mann et al Chembiochem. 2024 May 2;25(9):e202400007

    Insert

    Cry2 - mCherry - Profilin.WT (Cry2, PRF1 Mustard Weed, Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Profilin WT (Mus musculus)

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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