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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 2681 - 2720 of 8358 results

  1. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

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  2. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid
    #69058
    Purpose
    Dual Fluorescent Reporter for F-actin and Nuclei
    Depositor
    Olivier Pertz
    Insert
    LifeAct-GFP_IRES_NLS-mCherry
    Use
    Tags
    Lifeact-GFP, NLS-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69058-plasmid-map-sequence-id-123543

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  3. pGBScrispri Sham

    Plasmid
    #223201
    Purpose
    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223201-plasmid-map-sequence-id-451878

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  4. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  5. AbVec_IGHC

    Plasmid
    #183701
    Purpose
    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGHG1 (IGHG1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183701-plasmid-map-sequence-id-358532

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  6. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*40:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180463-plasmid-map-sequence-id-355082

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  7. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGKC (IGKC )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183702-plasmid-map-sequence-id-358531

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  9. AbVec_IGLC

    Plasmid
    #183703
    Purpose
    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGLC2 (IGLC2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183703-plasmid-map-sequence-id-358529

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  10. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  11. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

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  12. pTCF7 HLA-B*07:02

    Plasmid
    #180446
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*07:02
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180446-plasmid-map-sequence-id-355789

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  13. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Use
    Tags
    Expression
    Mammalian and Plant
    Mutation
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

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  14. pLIX403-EYFP-ccdB-Hygro

    Plasmid
    #158555
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158555-plasmid-map-sequence-id-309868

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  15. XLone-Puro eGFP

    Plasmid
    #140027
    Purpose
    Tunable and temporal expression control of nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140027-plasmid-map-sequence-id-275840

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  16. hSYN-mito-dsRED2

    Plasmid
    #173069
    Purpose
    mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Lentiviral
    Tags
    dsRED
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    173069-plasmid-map-sequence-id-345051

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  17. pLIX403-ccdB-G418

    Plasmid
    #158561
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158561-plasmid-map-sequence-id-309877

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  18. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110422-plasmid-map-sequence-id-213315

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  19. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

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  20. pmr101A-cPr-mCherry (KmR)

    Plasmid
    #177207
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177207-plasmid-map-sequence-id-348603

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  21. pCEV-G2-Km ymNeongreen

    Plasmid
    #193959
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast
    Depositor
    Bas Teusink
    Insert
    ymNeonGreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193959-plasmid-map-sequence-id-383894

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  22. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

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  23. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Use
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  24. pTCF5 HLA-A*01:01

    Plasmid
    #180448
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*01:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180448-plasmid-map-sequence-id-355109

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  25. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  26. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

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  28. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

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  29. RAB5AA-c003

    Plasmid
    #175475
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-N215.
    Depositor
    Opher Gileadi
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175475-plasmid-map-sequence-id-353172

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  30. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid
    #219828
    Purpose
    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219828-plasmid-map-sequence-id-441125

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  31. pLIX403-EYFP-ccdB-Zeo

    Plasmid
    #158565
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158565-plasmid-map-sequence-id-309880

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  32. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  33. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  34. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  35. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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  36. pD451-pA-APEX2

    Plasmid
    #157863
    Purpose
    Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling
    Depositor
    Alex Kentsis
    Insert
    pA-APEX2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    157863-plasmid-map-sequence-id-308764

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  37. BC-GPR56

    Plasmid
    #44197
    Depositor
    Lei Xu
    Insert
    human GPR56 (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    LTR
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44197-plasmid-map-sequence-id-59870

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  38. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  39. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72259-plasmid-map-sequence-id-131837

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  40. SPLICSFAST-LDh-MT-P2A

    Plasmid
    #214896
    Purpose
    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214896-plasmid-map-sequence-id-423482

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Showing: 2681 - 2720 of 8358 results