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We narrowed to 13,363 results for: BASE

Showing: 1341 - 1360 of 13363 results
  1. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. MyoUTipGAPi

    Plasmid
    #127549
    Purpose
    Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment
    Depositor
    Insert
    Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
    Use
    RNAi; Positive control
    Promoter
    Ubiquitin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pFP59

    Plasmid
    #247215
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from an E. coli sigma70 promoter. Suitable for cell-free expression.
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    Pj23100 (ttgacggctagctcagtcctaggtacagtgctagc)
    Available Since
    May 29, 2026
    Availability
    Academic Institutions and Nonprofits only
  4. pFP60

    Plasmid
    #247216
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from an E. coli sigma70 promoter. Suitable for cell-free expression.
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    Pj23100 (ttgacggctagctcagtcctaggtacagtgctagc)
    Available Since
    May 29, 2026
    Availability
    Academic Institutions and Nonprofits only
  5. pFP34

    Plasmid
    #247209
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from a T7 promoter. Suitable for cell-free expression.
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
  6. pFP34∆RBS

    Plasmid
    #247213
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from a T7 promoter but lacks a ribosomal binding site. Suitable for cell-free expression.
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
  7. pFP35∆RBS

    Plasmid
    #247214
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from a T7 promoter but lacks a ribosomal binding site. Suitable for cell-free expression.
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
  8. pYPQAT16

    Plasmid
    #223388
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.
    Depositor
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pYPQAT41

    Plasmid
    #223413
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.
    Depositor
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pYPQAT26

    Plasmid
    #223398
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.
    Depositor
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pYPQAT29

    Plasmid
    #223401
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.
    Depositor
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1341 - 1360 of 13363 results