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pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X

Plasmid

#147929

Purpose

Mammalian Expression of HsNanos2_6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2_6-22 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mApple

Plasmid

#191741

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mApple

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mCherry

Plasmid

#191742

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHWBF01

Plasmid

#131022

Purpose

Synthetic source clone for maize Histon H2B.5 with C-terminal mCherry fusion

Depositor

Hank Bass

Article

Howe et al DNA Cell Biol. 2012 Jun;31(6):925-38. do

Insert

Histone H2B.5

Use

Cloning vector

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNa-delta S

Plasmid

#107983

Purpose

bacterial expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNa 1080bp-17aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Use

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9

Plasmid

#120379

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hY

Plasmid

#60995

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

histone 2B tag

Expression

Mutation

Promoter

Bactin2

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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NTERY06

Plasmid

#184124

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY06

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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mNTERY00

Plasmid

#184110

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY00

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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UbG75C

Plasmid

#37194

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

Ubiquitin G75C mutant (RBX1 Human)

Use

Tags

No tag

Expression

Bacterial

Mutation

Glycine 75 changed to Cystine

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-mfR

Plasmid

#60991

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKate2

Use

Zebrafish

Tags

membrane tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mG

Plasmid

#60987

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Tags

membrane tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
p3E-ChBP

Plasmid

#108892

Purpose

Multisite gateway vector for 3' tagging with a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

ChBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9C104A

Plasmid

#120380

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

C321A

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hR

Plasmid

#60997

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

histone 2B tag

Expression

Mutation

Promoter

Bactin2

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Use

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_HR_Prnp_3

Plasmid

#78621

Purpose

pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

pX330_HR_Prnp_3

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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