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pcDNA3.1-TIE-V5/HIS

Plasmid

#205612

Purpose

Expression of human TIE receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TIE receptor tyrosine kinase (TIE1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD1 Flag

Plasmid

#74893

Purpose

Express COMMD1 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD1 (COMMD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBR322-MCK1.35lux

Plasmid

#53368

Purpose

expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

Depositor

Josephine Nalbantoglu

Article

Larochelle et al Gene Ther. 1997 May;4(5):465-72.

Insert

muscle creatine kinase promoter/enhancer 1.35kb

Expression

Mammalian

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pEBB COMMD10 Flag

Plasmid

#74902

Purpose

Express COMMD10 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD10 (COMMD10 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

Plasmid

#40807

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(44-51), S218E, S222D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
PB_EF1a-CasRx-msfGFP-2A-Blast

Plasmid

#226010

Purpose

CasRx protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

CasRx RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoA

Plasmid

#220916

Purpose

Neuronal specific expression of AldoA

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoA (ALDOA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD5 Flag

Plasmid

#74897

Purpose

Express COMMD5 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD5 (COMMD5 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD2 Flag

Plasmid

#74894

Purpose

Express COMMD2 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD2 (COMMD2 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip

Plasmid

#178054

Purpose

myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK14_HUMAN_D0

Plasmid

#79680

Purpose

This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK14

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEBB COMMD9 Flag

Plasmid

#74901

Purpose

Express COMMD9 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD9 (COMMD9 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD8 Flag

Plasmid

#74900

Purpose

Express COMMD8 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD8 (COMMD8 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pB.FRB-3myc-KLC1a

Plasmid

#134627

Purpose

Mammalian expression of mouse KLC1a

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KLC1a (Klc1 Mouse)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag B)

Plasmid

#16606

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag B) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragB (KpnI->AccI). The KpnI site is at -1194 …

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only