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SIRT6 Flag

Plasmid

#13817

Purpose

Mammalian expression of human SIRT6 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT6 (SIRT6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-CMV

Plasmid

#167500

Purpose

Expression of dual inducible Cas9 with CMV promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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nuclear cpVenus control

Plasmid

#186790

Purpose

Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

Orai1-GCaMP6f (ORAI1 Human, Synthetic)

Tags

GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma

Plasmid

#8895

Purpose

Mammalian expression of flag-tagged PPAR gamma

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

Plasmid

#154868

Purpose

Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

Depositor

Ulrik Gether

Has Service

AAV Retrograde and AAV8

Insert

hM3D(Gq)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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