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iLID-mCl3-MAP7N

Plasmid

#190167

Purpose

Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

iLID-mClover3-MAP7N

Use

Tags

iLID-mClover3

Expression

Mammalian

Mutation

aa 1-309 from human MAP7 delta175-213

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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Stargazin-mCherry-FRB

Plasmid

#172443

Purpose

Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Use

Tags

mCherry-HA-FRB

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Puro

Plasmid

#227259

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Tags

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Mutation

Promoter

Hsp83

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

Plasmid

#85427

Purpose

Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-DIO-axon-jGCaMP8f

Plasmid

#220531

Purpose

For Cre-dependent enriched expression of GCaMP8f in axons

Depositor

Lin Tian

Insert

Axon-DIO-GCaMP8f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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