We narrowed to 17,366 results for: puro

1. pLKO.1-puro-cGASsh4

   Plasmid

   #127646

   Purpose

   Knock-down of human cGAS

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   cGAS shRNA (CGAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-DNMT3A-PuroR_hNT

   Plasmid

   #71830

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   Non-targeting sgRNA human (DNMT3A Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSIIpuro-AREG-ScNeo

   Plasmid

   #209897

   Purpose

   To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   AREG-ScNeo (AREG Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Puro-mScarlet

   Plasmid

   #207689

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. VRK1-dTag-puro

   Plasmid

   #199651

   Purpose

   Expresses VRK1 C-term. Tagged with dTAG

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   dTAG

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-3xFLAG-Puro

   Plasmid

   #207728

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-mNeon-Puro

   Plasmid

   #207720

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-CCNE1-HA-puro

   Plasmid

   #212669

   Purpose

   Express tagged cyclin E1

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   CCNE1 (CCNE1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-puro GFP-b5 ER

   Plasmid

   #38274

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   Cytochrome b5 (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Cyb5a Tranmembrane region from 95 to 134 amino ac…

   Promoter

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. CROPseq-Puro-24xMS2

    Plasmid

    #212629

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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