We narrowed to 2,361 results for: Efs
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pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113700PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113698PurposeDox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_EEF1A1_GTP-EFTU-D2
Plasmid#109811PurposeProtein expression and purification of EEF1A1_GTP-EFTU-D2DepositorInsertEEF1A1_GTP-EFTU-D2 (EEF1A1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p23-mNeongreen-DDX21 HGV
Plasmid#179230Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreenExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT
Plasmid#179229Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreenExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-92a-1-5p
Plasmid#103750PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-92a-1-5p target (MIR92A1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLINK cyclin T1 (1-280) myc (all 200) (P#575)
Plasmid#14610DepositorInsertcyclin T1 (1-280) (all 200) (CCNT1 Human)
UseTagsmycExpressionMammalianMutationC198A, C200A, H202A, C205A. Active in 3T3 cells.PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Tet-Off-FLEX-DEST (JDW 1186)
Plasmid#229820PurposeAn AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTADepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)
Plasmid#229824PurposeA PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tetDepositorInsertLuciferase-P2A-H2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only