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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Plasmid

#176625

Purpose

TF A self-activation cassette in MultiFate-2.1

Depositor

Michael Elowitz

Article

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_SIX1_P2A_Hygro_Barcode

Plasmid

#120476

Purpose

Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SIX1 (SIX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-C-CARMIL2b

Plasmid

#118741

Purpose

To express CARMIL2 with GFP tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID5B

Plasmid

#86288

Purpose

Donor vector for 3' FLAG tag of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID5B homology arms (ARID5B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K310R

Plasmid

#196630

Purpose

Express GFP-RELA K310R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K310R (RELA Human)

Use

Lentiviral

Tags

GFP

Mutation

K310R

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_1

Plasmid

#86307

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJET1.2-U3

Plasmid

#173156

Purpose

Single guide RNA cassette under U3 promoter

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Insert

sgRNA cassette under U3 promoter

Expression

Plant

Promoter

promoter region of the U3C snRNA gene (Marshallsa…

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_MIER3

Plasmid

#86274

Purpose

Donor vector for 3' FLAG tag of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIER3 homology arms (MIER3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROG1_P2A_Hygro_Barcode

Plasmid

#120467

Purpose

Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_POU1F1_P2A_Hygro_Barcode

Plasmid

#120473

Purpose

Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU1F1 (POU1F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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