We narrowed to 7,880 results for: Lif

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS5

Plasmid

#111760

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS5-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GFP-2A-SynmRuby

Plasmid

#166608

Purpose

Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GFP-2A-syn

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-mito

Plasmid

#113916

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Plasmid

#87644

Purpose

For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::SNAPf>myr::CLIPf>myr::Halo2

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Article

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-SADB19_oG-IRES-Puro

Plasmid

#172367

Purpose

Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oG + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pO40

Plasmid

#128902

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab6AQL-BirA*-HA-MAO (RAB6A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB4

Plasmid

#128903

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab6ATN-BirA*-HA-MAO (RAB6A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETM11-6xHis-TEV-hORF1p_R261A

Plasmid

#202586

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Arginine 261 to Alanine

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Promoter

tetracycline response element

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT005-HaloGPM

Plasmid

#163385

Purpose

2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Txnip(C247S)

Plasmid

#206353

Purpose

AAV plasmid expressing mutant TXNIP

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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optoAKT

Plasmid

#207164

Purpose

Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

Depositor

Kai Zhang

Article

Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

Insert

CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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