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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2C-ΔC-S230A

Plasmid

#200561

Purpose

Secreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab SARS-CoV-2, Synthetic)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DIO-PinkyCaMP

Plasmid

#232857

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

Plasmid

#169182

Purpose

Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Tags

His6-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65453

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV1

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-nsp13 (SARS-CoV-2)

Plasmid

#169190

Purpose

Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)

Tags

GST

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.10 EXOC3prom SV40

Plasmid

#246713

Purpose

To investigate the influence of a universal enhancer (SV40) on EXOC3 promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

EXOC3 Promoter (EXOC3 Human)
SV40 universal enhancer

Use

Luciferase

Mutation

WT

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 MIDDLE 300BP

Plasmid

#226440

Purpose

For subcloning of human EXOC3 promoter (middle 300 bp) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 MIDDLE 300BP (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 3' HALF

Plasmid

#226439

Purpose

For subcloning of human EXOC3 promoter (3' half) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 3' HALF (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-EXOC3 5' HALF

Plasmid

#226438

Purpose

For subcloning of human EXOC3 promoter (5' half) or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 5' HALF (EXOC3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

Plasmid

#240228

Purpose

Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

Plasmid

#65429

Purpose

Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Mutation

H134R variant

Promoter

hSyn1

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

Plasmid

#65454

Purpose

Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Mutation

H134R variant

Promoter

hSyn1

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pADC10-SETXhel

Plasmid

#193056

Purpose

Baculovirus expression of human Senataxin helicase domain for protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Senataxin (SETX Human)

Tags

Flag

Expression

Insect and Mammalian

Mutation

codon optimized for insect cell expression

Promoter

hybrid p10 (insect) and CMV (human) expression

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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