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We narrowed to 5,036 results for: U6...

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Showing: 1361 - 1380 of 5036 results

  2. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  3. pY036_ATP1A1_G3

    Plasmid
    #86617
    Purpose
    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86617-plasmid-map-sequence-id-168852

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  4. sgRNA.SFFV.tRFP

    Plasmid
    #169941
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169941-plasmid-map-sequence-id-338357

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  5. sgRNA.SFFV.tBFP

    Plasmid
    #169940
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169940-plasmid-map-sequence-id-338359

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  6. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

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  7. sgRNA.SFFV.GFP

    Plasmid
    #169938
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169938-plasmid-map-sequence-id-338362

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  8. sgRNA.SFFV.RFP657

    Plasmid
    #169939
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and RFP657 from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169939-plasmid-map-sequence-id-338364

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  9. pShuttle-CMV-GFP U6promoter

    Plasmid
    #128049
    Purpose
    Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128049-plasmid-map-sequence-id-255046

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  10. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49157-plasmid-map-sequence-id-72446

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  11. pTJK644

    Plasmid
    #138012
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138012-plasmid-map-sequence-id-275045

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  12. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  13. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  14. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  15. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  16. pTJK635

    Plasmid
    #138009
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138009-plasmid-map-sequence-id-275004

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  17. pTJK643

    Plasmid
    #138011
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA1 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138011-plasmid-map-sequence-id-275015

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  18. pTJK642

    Plasmid
    #138013
    Purpose
    SIX1 CRISPRi
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA13 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138013-plasmid-map-sequence-id-275062

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  19. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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Showing: 1361 - 1380 of 5036 results