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PINK1-SPARK

Plasmid

#248086

Purpose

PINK1 phase separation-based biosensor

Depositor

Danielle Schmitt

Article

Vineall et al bioRxiv [Preprint]. 2025 Oct 16:2025.10.

Insert

PINK1-SPARK

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-IVT

Plasmid

#215834

Purpose

Template for in vitro transcription of CBE6b (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Article

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Promoter

T7

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AKAR4

Plasmid

#61620

Purpose

Lipid raft-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

Lyn-AKAR4

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a-IVT

Plasmid

#215833

Purpose

Template for in vitro transcription of CBE6a (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAS1-Prig-3::tagBFP

Plasmid

#165598

Purpose

rig-3 promoter derive BFP expression in AVA head neurons.

Depositor

Andrew Leifer

Article

Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

Insert

Prig-3, tagBFP

Expression

Worm

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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JD638

Plasmid

#160395

Purpose

Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis miR396-resistant GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEutRwc

Plasmid

#216334

Purpose

An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

Depositor

Mark Styczynski

Article

McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

Inserts

EutR
Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1hisA cdc25c C330S C377S

Plasmid

#10965

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

C330S C377S. Cysteine to Serine substitution at …

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Halo-PINK1-SPARK

Plasmid

#248088

Purpose

HaloTag labeled PINK1-SPARK

Depositor

Danielle Schmitt

Article

Vineall et al bioRxiv [Preprint]. 2025 Oct 16:2025.10.

Insert

Halo-PINK1-SPARK

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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PINK1-SPARK S/A

Plasmid

#248087

Purpose

PINK1-SPARK control construct with PINK1 phosphosite mutated

Depositor

Danielle Schmitt

Article

Vineall et al bioRxiv [Preprint]. 2025 Oct 16:2025.10.

Insert

PINK1-SPARK S/A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-PINK1-SPARK S/A

Plasmid

#248089

Purpose

HaloTag labeled PINK1-SPARK control construct with PINK1 phosphosite mutated

Depositor

Danielle Schmitt

Article

Vineall et al bioRxiv [Preprint]. 2025 Oct 16:2025.10.

Insert

Halo-PINK1-SPARK S/A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS-C312S

Plasmid

#115263

Purpose

For in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Mutation

C312S

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS-N72A-N199A

Plasmid

#115264

Purpose

For in vitro transcription of human NODAL open reading frame (with two mutated N-glycosylation sites) RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Mutation

N72A N199A

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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