We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. pYZ300

   Plasmid

   #102689

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp his3 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ293

   Plasmid

   #102687

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ292

   Plasmid

   #102686

   Purpose

   S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AIO-Puro

   Plasmid

   #74630

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Puromycin resistant marker

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDG458

   Plasmid

   #100900

   Purpose

   SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. KO plasmid

   Plasmid

   #135970

   Purpose

   Expresses SpCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT30

    Plasmid

    #204294

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Use

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJAT21

    Plasmid

    #204309

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAT27

    Plasmid

    #204308

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAT18

    Plasmid

    #204307

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJAT23

    Plasmid

    #204312

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAT22

    Plasmid

    #204311

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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