We narrowed to 2,303 results for: dap

1. pMRX-IPU-GFP-NEK9W967A

   Plasmid

   #168271

   Purpose

   Expresses LIR (LC3-interacting region) mutant NEK9

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 979 to alanine

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ABKAR-Kras

   Plasmid

   #84637

   Purpose

   Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, C…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZRSpBMN-­Z‐GFP‐Eps8

   Plasmid

   #74935

   Purpose

   Expresses mouse Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Intersectin I SH3 D domain (human)

   Plasmid

   #47416

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain D (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain D

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Intersectin I SH3 E domain (human)

   Plasmid

   #47417

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   ITSN1 intersectin 1 (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain E

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-ERKsub(TA)DD-Venus-NES

   Plasmid

   #84631

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ERKsub(TA)DD-Venus-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Mutated target Thr residue to Ala.

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-AMPKsub-YPet-Kras

   Plasmid

   #84638

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-Kras
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK-flag-USP19 494-1216 (USP)

   Plasmid

   #78588

   Purpose

   Expression flag-USP19 494-1216 (USP) in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 494-1216 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-493 and mutated 1216 residue…

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3-myc- mCE(Del 25C)

   Plasmid

   #82472

   Purpose

   Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   myc-mCE-C terminal deleted (Rngtt Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-LKB1(S431A)

    Plasmid

    #84641

    Purpose

    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(S431A) (STK11 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    PKA phosphorylation site Serine 428 mutated to Al…

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. H1x

    Plasmid

    #218111

    Purpose

    Encodes mus musculus histone 1 in Gateway entry vector

    Depositor

    Peter Hamilton

    Article

    Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

    Insert

    Histone H1x (H1f10 Mouse)
    

    Use

    Gateway cloning entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALN132

    Plasmid

    #239799

    Purpose

    Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain

    Depositor

    Iain Cheeseman, Ally Nguyen

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    MmCENPT--Rn-Histone Fold Domain
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney Murine Leukemia Virus (MMLV) LTR promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-MmCENP-W

    Plasmid

    #237433

    Purpose

    Expresses Mus musculus CENP-W; untagged; made for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-W (Cenpw Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-MmCENPT-GFP

    Plasmid

    #237426

    Purpose

    Expresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. act-3p(long):rfp

    Plasmid

    #202330

    Purpose

    The act-3 promoter drives expression of TurboRFP in the pharynx, spermatheca, and body wall of C. elegans.

    Depositor

    Didier Stainier

    Article

    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

    Inserts

    act-3p(long) promoter
    TurboRFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    This is a promoter sequence for C. elegans act-3 …

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid

    #217319

    Purpose

    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

    Depositor

    Noboru Mizushima

    Article

    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

    Insert

    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    

    Use

    Retroviral

    Tags

    HaloTag and SNAP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHC024 (∆SH)

    Plasmid

    #226202

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for soluble hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxFUYHWI ∆hypA2B2F2

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCHC023 (∆MBH)

    Plasmid

    #226201

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for membrane bound hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCHC022 (∆phcA)

    Plasmid

    #226200

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge