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pCAG-hPPP3CA-FLAG

Plasmid

#179134

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb-4Rs

Plasmid

#165022

Purpose

expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb-4Rs (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

change lysine residues on position 109, 135, 151,…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLX302_HOXB13

Plasmid

#70089

Purpose

Expression of human HOXB13 from CMV promoter, puromycin selection marker

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Crimson

Plasmid

#70683

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

S. pyogenes sgRNA cassette
Crimson Reporter

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-a and hU6

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE3G-mouse cGAS-HA

Plasmid

#186875

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS with C-terminal HA tag (Cgas Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wt-FLAG

Plasmid

#204525

Purpose

Retroviral expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHCD1C

Plasmid

#102954

Purpose

gene cloning of human CD1C

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

Insert

CD1C cDNA (CD1C Human)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-actin prom-dsRed-adra2a.1227.shRNA

Plasmid

#67880

Purpose

expresses dsRed and shRNA to knock down adra2a receptors

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

dsRed and shRNA to knock down the mouse adra2 receptor

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5

Plasmid

#214701

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52

Plasmid

#214700

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wt

Plasmid

#214699

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR wt (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192789

Purpose

aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody minimizer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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