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p2CT-His-MBP-Lbu_C2c2_K310A

Plasmid

#91856

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K310A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K310A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_D1078A

Plasmid

#91860

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) D1078A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

D1078A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_N314A

Plasmid

#91858

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) N314A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

N314A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1080A

Plasmid

#91862

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1080A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA6

Plasmid

#83869

Purpose

Gateway donor vector for IAA6

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA6 (IAA6 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA7_no stop

Plasmid

#83872

Purpose

Gateway donor vector for IAA7 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA7 (IAA7 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA3

Plasmid

#83862

Purpose

Gateway donor vector for IAA3

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA20_no stop

Plasmid

#83859

Purpose

Gateway donor vector for IAA20 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA20 (IAA20 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA19_no stop

Plasmid

#83857

Purpose

Gateway donor vector for IAA19 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA19 (IAA19 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA5

Plasmid

#83867

Purpose

Gateway donor vector for IAA5

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_173011.1 (IAA5 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA31_no stop

Plasmid

#83865

Purpose

Gateway donor vector for IAA31 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_188387.1 (IAA31 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA2_no stop

Plasmid

#83858

Purpose

Gateway donor vector for IAA2 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA2 (IAA2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA19

Plasmid

#83856

Purpose

Gateway donor vector for IAA19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA19 (IAA19 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA14_no stop

Plasmid

#83852

Purpose

Gateway donor vector for IAA14

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA14 (IAA14 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA14

Plasmid

#83851

Purpose

Gateway donor vector for IAA14

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA14 (IAA14 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA13_no stop

Plasmid

#83850

Purpose

Gateway donor vector for IAA13 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA13 (IAA13 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA12_no stop

Plasmid

#83849

Purpose

Gateway donor vector for IAA12 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NM_100334.4 (IAA12 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Article

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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