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ND2-FusXTBE-Left TALE

Plasmid

#196498

Purpose

Expresses left-side TALE-DddAtox Cterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Right TALE

Plasmid

#196499

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-Left TALE

Plasmid

#196500

Purpose

Expresses left-side TALE-DddAtox Cterm half Cox1-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-Cox1 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-Right TALE

Plasmid

#196501

Purpose

Expresses Right-side TALE-DddAtox Nterm half Cox1-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-Cox1 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-Left TALE

Plasmid

#196502

Purpose

Expresses left-side TALE–DddAtox N term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 left TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tl1-FusXTBE-Left TALE

Plasmid

#196506

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 left TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tl1-FusXTBE-RightTALE

Plasmid

#196507

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dimer_mKate2

Plasmid

#167333

Purpose

Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K95A, Y99F, E106A, E112A and R116A

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dC_mKate2

Plasmid

#167336

Purpose

Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_L6_mKate2

Plasmid

#167332

Purpose

Expresses mKate2 fused to TFAM L6 mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K136, H137, K139, R140, K146 and K147 to …

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4*

Plasmid

#71402

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4* (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

After aa126, insertion leads to reading frame shi…

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-L108S/L109S/F112S

Plasmid

#48730

Purpose

Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 and 109 to Serine; phenylalanine 112 …

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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iYFP-Parkin Plasmid

Plasmid

#197107

Purpose

Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

Depositor

Dori Woods

Article

Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

Insert

parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)

Use

Retroviral

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Use

Tags

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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