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Showing: 1361 - 1380 of 79164 results

  3. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51760-plasmid-map-sequence-id-392672

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  5. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60621-plasmid-map-sequence-id-208224

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  19. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62889-plasmid-map-sequence-id-105912

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  20. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

    Plasmid
    #51509
    Purpose
    Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Hongkui Zeng
    Publication
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Has service
    AAV1
    Insert
    tdTomato-T2A-SypEGFP
    Use
    AAV
    Promoter
    phSyn1
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51509-plasmid-map-sequence-id-425635

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Showing: 1361 - 1380 of 79164 results