We narrowed to 11,550 results for: SHA

1. pcdna3 flag - ULK2 wt

   Plasmid

   #27637

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2010 Dec 23. ():.

   Insert

   mouse ULK2 (Ulk2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Initial M removed from ULK2.

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pLV-RhoB Sensor-IRES-Blast

   Plasmid

   #222633

   Purpose

   Rho reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   RhoB Sensor
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-V5-DGCR8-mDRBD1&2

   Plasmid

   #51385

   Depositor

   Narry Kim

   Article

   Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-V5-DGCR8-mDRBD1

   Plasmid

   #51384

   Depositor

   Narry Kim

   Article

   Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   DRBD1 mutant (A568K, A569K)

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-V5-DGCR8-mDRBD2

   Plasmid

   #51386

   Depositor

   Narry Kim

   Article

   Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   DRBD2 mutant (A676K, S677K)

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pNEU-KlacRS

   Plasmid

   #232155

   Purpose

   Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   KlacRS1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-mCherry-TAG-EGFP

   Plasmid

   #232156

   Purpose

   A fluorescence reporter to show incorporation of target unnatural amino acids.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   mCherry-linker(TAG)-EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEvol-MmKlacRS1

   Plasmid

   #232157

   Purpose

   Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   KlacRS1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEvol-chKlacRS-WT

   Plasmid

   #232159

   Purpose

   A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   chKlacRS-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEvol-chKlacRS-IPYE

    Plasmid

    #232160

    Purpose

    A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    chKlacRS-IPYE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NmMetQ

    Plasmid

    #172111

    Purpose

    Expression of Methionine Binding Protein (MetQ) with signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-miRFP680-NES-p2A-dTomato-2xrGBD

    Plasmid

    #222638

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-dTomato-2xrGBD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

    Plasmid

    #222634

    Purpose

    Rho reporter, sensor dead

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    RhoB Sensor (PKN-L59Q)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PKN L59Q

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TRE-mClover3

    Plasmid

    #214922

    Purpose

    expression vector control - contitutive expression of mClover3 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mClover3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRE-mRuby3

    Plasmid

    #214923

    Purpose

    expression vector control - contitutive expression of mRuby33 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-5X2-hDrp1(518-736)-Flag

    Plasmid

    #37244

    Depositor

    David Kashatus, Christopher Counter

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    contains AA 517-end

    Promoter

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-mRuby3-MLC-IRES-Neo

    Plasmid

    #222635

    Purpose

    Reporter for activated NM2

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mRuby3-MLC (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRE-SRRD-mRuby3

    Plasmid

    #214920

    Purpose

    inducible expression of SRRD tagged on C-terminus with mRuby3

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a-SRRD-noTag (in pLEX_307)

    Plasmid

    #214919

    Purpose

    constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EF1a-SRRD-HA (in pLEX_307)

    Plasmid

    #214918

    Purpose

    constitutive expression of SRRD tagged with HA

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NmMetQ C20A

    Plasmid

    #172112

    Purpose

    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine on MetQ and Cystein…

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-TREtight-mTagBFP2-B19G

    Plasmid

    #100799

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Plasmid

    #100798

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    TVA, EGFP, tet transactivator
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcdna6.2- myc ULK1 wt

    Plasmid

    #27629

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pDONR221 - myc ULK1 wt

    Plasmid

    #27623

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

28. MKC177_CCR5(PGK-synEPOR)

    Plasmid

    #232415

    Purpose

    AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MKC176_EPOR(2A-synEPOR)

    Plasmid

    #232414

    Purpose

    AAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a-SRRD-V5-APEX2 (in pLEX_307)

    Plasmid

    #214921

    Purpose

    constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcdna6.2- myc ULK1 k46I

    Plasmid

    #27630

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pcdna6.2- myc ULK1 4SA

    Plasmid

    #27631

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-mCherry-FKBP-GEF(ITSN1)

    Plasmid

    #222631

    Purpose

    Cdc42 activation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQCXIN- myc ULK1 wt

    Plasmid

    #27626

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pDONR221 - myc ULK1 4SA

    Plasmid

    #27625

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

37. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

    Plasmid

    #232412

    Purpose

    AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MKC175_HBA1reg(synEPOR)

    Plasmid

    #232413

    Purpose

    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

    Plasmid

    #222636

    Purpose

    Ezrin activation reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQCXIN- myc ULK1 4SA

    Plasmid

    #27628

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only