We narrowed to 475 results for: piggybac vectors

1. pB-Ric8b-Integration

   Plasmid

   #129457

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   Ric8b (Ric8b Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB-RTP2-Integration

   Plasmid

   #129459

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP2 (RTP2 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-EF1a-NLuc-IRES-Puro

   Plasmid

   #130936

   Purpose

   PiggyBac vector for constitutive NanoLuc expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BII-ChBtW-iresFUCCI

   Plasmid

   #133371

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-CT3G-ERP2-MG

   Plasmid

   #175501

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-T3G-CERP2

   Plasmid

   #175498

   Purpose

   All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-CT3G-CERP2

   Plasmid

   #175499

   Purpose

   All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BII-Sh-PnGW

    Plasmid

    #133359

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-T3G-ERP2

    Plasmid

    #175188

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-CT3G-ERP2

    Plasmid

    #175497

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BII-ChPtW-PLAGL2

    Plasmid

    #133380

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    PLAGL2 (PLAGL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-PtH

    Plasmid

    #133391

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BII-C3HTK

    Plasmid

    #133388

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BII-ChBtW-eSpCas9

    Plasmid

    #133370

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BII-ChBtW-PLAGL2

    Plasmid

    #133369

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    PLAGL2 (PLAGL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-BtH

    Plasmid

    #133392

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YX022 (PB)EF1a-LIDARsensor (hygR)

    Plasmid

    #233431

    Purpose

    EF1a-driven LIDAR reporter RNA

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BII-Sh-PnGW-CMV

    Plasmid

    #133366

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BII-ChPtW-eSpCas9

    Plasmid

    #133381

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BII-Sh-PnGW-CMV-Luc

    Plasmid

    #133367

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BII-Sh-PIR702W-Luc

    Plasmid

    #133362

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BII-Sh-PIR702W

    Plasmid

    #133361

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BII-Sh-PnGW-Luc

    Plasmid

    #133360

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pB-MultiplexedReporter-MOR170-1

    Plasmid

    #129461

    Purpose

    piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    MOR170-1 (Olfr895 Mouse)
    

    Use

    Synthetic Biology

    Tags

    LUCY tag, Rho tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pB-RTP1S-Integration

    Plasmid

    #129458

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP1 (Rtp1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the first 36 amino acids of the protein

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

    Plasmid

    #179585

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NLS-NG-T2A

    Expression

    Mutation

    Promoter

    CAGp

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BII-Sh-FUCCI

    Plasmid

    #133363

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPB-PNGT3-d2EGFP_CMV-mCherry

    Plasmid

    #213765

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

    Insert

    PNGT#3-d2EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAC150-RFP-EGFP-LGALS3

    Plasmid

    #175778

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid

    #175509

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AgDD

    Plasmid

    #78289

    Purpose

    create protein aggregates in living cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge