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Showing: 301 - 350 of 562 results

  1. MCHR1-DuET

    Plasmid
    #213337
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR1 (MCHR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213337-plasmid-map-sequence-id-430912

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  5. TFORF2901

    Plasmid
    #144161
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144161-plasmid-map-sequence-id-396224

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  6. TFORF2902

    Plasmid
    #143658
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143658-plasmid-map-sequence-id-400440

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  7. TFORF2904

    Plasmid
    #143388
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143388-plasmid-map-sequence-id-400329

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  8. TFORF2903

    Plasmid
    #143659
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143659-plasmid-map-sequence-id-400444

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  9. TFORF3060

    Plasmid
    #144536
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BACH1 (BACH1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144536-plasmid-map-sequence-id-404255

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  10. TFORF0757

    Plasmid
    #143074
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NOTCH1 (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143074-plasmid-map-sequence-id-399050

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  18. pDONR221-SLC14A1_STOP

    Plasmid
    #161123
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC14A1 (SLC14A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161123-plasmid-map-sequence-id-334661

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  20. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  21. pLPC-dnMAML1-mVenus

    Plasmid
    #171023
    Purpose
    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    MAML1 Dominant Negative (MAML1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    amino acids 12-74 only, hence conferring dominant…
    Promoter
    CMV
    Available since
    April 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    171023-plasmid-map-sequence-id-336789

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  25. mEmerald-Occludin-N-10

    Plasmid
    #54212
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. mEmerald-Occludin-C-14

    Plasmid
    #54211
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54211-plasmid-map-sequence-id-140287

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  27. CAG-MCSrev-WPRE-SV40 - GCH4

    Plasmid
    #230832
    Purpose
    AAV cloning vector for expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230832-plasmid-map-sequence-id-459148

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  28. TRE-MCS-WPRE-SV40 - GCH25

    Plasmid
    #230826
    Purpose
    AAV cloning vector for expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230826-plasmid-map-sequence-id-459123

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  29. Syn-MCS-WPRE-SV40 - GCH7

    Plasmid
    #230817
    Purpose
    AAV cloning vector for expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230817-plasmid-map-sequence-id-457138

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  30. GFAP-MCS-WPRE-SV40 - GCH31

    Plasmid
    #230829
    Purpose
    AAV cloning vector for expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230829-plasmid-map-sequence-id-457134

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  31. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  32. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  33. pDUAL OCLN (GFP)

    Plasmid
    #86982
    Purpose
    Lentiviral expression construct encoding Occludin and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    OCLN (OCLN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86982-plasmid-map-sequence-id-173293

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  34. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Tags
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. tdEos-Occludin-N-10

    Plasmid
    #57651
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid
    #230815
    Purpose
    AAV cloning vector for CRE dependent expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230815-plasmid-map-sequence-id-466637

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  37. CAG-FRT-MCS-WPRE-SV40 - GCH3

    Plasmid
    #230816
    Purpose
    AAV cloning vector for Flpo dependent expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230816-plasmid-map-sequence-id-466625

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  38. LT3GEPIR-MSH2shRNA1

    Plasmid
    #220587
    Purpose
    To inducibly knockdown MSH2 expression
    Depositor
    Sean Lee
    Insert
    MSH2 shRNA1 (MSH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220587-plasmid-map-sequence-id-437724

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  39. mEmerald-PINCH-N-14

    Plasmid
    #54230
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    PINCH (LIMS1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mEmerald-PINCH-C-14

    Plasmid
    #54229
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    PINCH (LIMS1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. PBRM1

    Plasmid
    #39013
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. PBRM1

    Plasmid
    #39103
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. PBRM1

    Plasmid
    #39027
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  44. PBRM1

    Plasmid
    #38999
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. mPlum-MCHR1-N-10

    Plasmid
    #55987
    Purpose
    Localization: Cilium, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    MCHR1 (Mchr1 Mouse)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. PBRM1

    Plasmid
    #39028
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. PBRM1

    Plasmid
    #39030
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pcDNA3.1-MS2-HA-HsRRP45_Z

    Plasmid
    #148133
    Purpose
    Mammalian Expression of HsRRP45
    Depositor
    Elisa Izaurralde
    Insert
    HsRRP45 (EXOSC9 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148133-plasmid-map-sequence-id-290581

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  49. pT7-V5-SBP-C1-HsRRP45_Z

    Plasmid
    #148143
    Purpose
    Mammalian Expression of HsRRP45
    Depositor
    Elisa Izaurralde
    Insert
    HsRRP45 (EXOSC9 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148143-plasmid-map-sequence-id-290591

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  50. pcDNA3-Casp2-Flag

    Plasmid
    #11811
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 (CASP2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11811-plasmid-map-sequence-id-299764

    Enlarge

Showing: 301 - 350 of 562 results