Skip to main content
Addgene
Showing: 121 - 140 of 268 results
  1. Tac(5A)-GFP

    Plasmid
    #162495
    Purpose
    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. GFP-Tac(5A)

    Plasmid
    #162493
    Purpose
    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. GFP-Tac-STC

    Plasmid
    #162490
    Purpose
    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Insert
    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pIN3_enpp-1_mScarlet

    Plasmid
    #182934
    Purpose
    Expresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)
    Depositor
    Insert
    genomic enpp-1 fused to mScarlet
    Use
    Tags
    flexible linker (GGGGS)x3 and mScarlet tag
    Expression
    Worm
    Mutation
    Promoter
    enpp-1
    Available since
    Sept. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Insert
    FcD265A-FLAG
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pSin-EF2-DMD53-GFP

    Plasmid
    #138353
    Purpose
    Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences
    Depositor
    Insert
    DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    EF2
    Available since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. GFP-Cstem-Tac(5A)

    Plasmid
    #162497
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).
    Depositor
    Insert
    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 268 results