We narrowed to 157 results for: SPT

1. pCMV-calpainsensor

   Plasmid

   #36182

   Purpose

   detection of activity of ubiquitous calpains

   Depositor

   Isabelle Richard

   Article

   Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

   Insert

   alpha-Fodrin cleavage site (Sptan1 Mouse)
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASP-3G124-HilA

   Plasmid

   #153324

   Purpose

   Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   3G124 DARPin but no expression
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAVA-LGF2-Gal11p

   Plasmid

   #127481

   Purpose

   Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

   Depositor

   Joel Malek

   Article

   Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

   Insert

   LGF2-Gal11p (GAL11 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Affibody EGFR:1907.BirA*-pET301/NT-DEST

   Plasmid

   #174691

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAVA-Gal11p-LGF2

   Plasmid

   #127480

   Purpose

   Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction

   Depositor

   Joel Malek

   Article

   Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

   Insert

   Gal11p-LGF2 (GAL11 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GvpA-CaM

   Plasmid

   #223555

   Purpose

   Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.

   Depositor

   Jean Gariepy

   Article

   Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

   Insert

   pET300-GvpACaM
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HiBiT-FZD8

   Plasmid

   #195846

   Purpose

   Expresses human FZD8 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD8 (FZD8 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HiBiT-FZD1

   Plasmid

   #195839

   Purpose

   Expresses human FZD1 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD1 (FZD1 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDEF3‐hTIM3 (no tag)

    Plasmid

    #49212

    Purpose

    expresses human Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (HAVCR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MC4R

    Plasmid

    #233630

    Purpose

    encodes the human melanocortin 4 receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    MC4R (MC4R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HiBiT-FZD5

    Plasmid

    #195843

    Purpose

    Expresses human FZD5 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD5 (FZD5 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HiBiT-FZD3

    Plasmid

    #195841

    Purpose

    Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD3 (FZD3 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    I292V and V495I

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MFE23-ZZ

    Plasmid

    #223553

    Purpose

    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

    Depositor

    Jean Gariepy

    Article

    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

    Insert

    pET302-MFE23-ZZ
    

    Use

    Tags

    HA tag and HIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid

    #213728

    Purpose

    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HiBiT-FZD4

    Plasmid

    #195842

    Purpose

    Expresses human FZD4 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge