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Plasmid#128447Purposelentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoterDepositorInsertEf1a promoter, C-terminal HA tag, and SV40 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_8.2_M3_CAMKIIA-gateway-V5-SV40
Plasmid#128462Purposelentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, and SV40 promoterDepositorInsertCAMKIIA promoter, C-terminal V5 tag, and SV40 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2+IA
Plasmid#111418Purposeexpress murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArA WT
Plasmid#59804PurposeAllows the integration of Myc ArA in the genome and Tet-inducible expressionDepositorInsertAurora A (AURKA Human)
UseTagsMycExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K403A
Plasmid#195612PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA1463G, A1464CPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArA K162R
Plasmid#59805PurposeAllows the integration of Myc ArA K162R in the genome and Tet-inducible expressionDepositorInsertAurora A (AURKA Human)
UseTagsMycExpressionMammalianMutationK162R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArB WT RNAiR
Plasmid#59806PurposeAllows the integration of Myc ArB in the genome and Tet-inducible expression.DepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationRNAi resistantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 TO FRT Myc ArB K106R
Plasmid#59807PurposeAllows the integration of Myc ArB K106R in the genome and Tet-inducible expressionDepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationK106R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo
Plasmid#128463Purposelentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertCAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo
Plasmid#128452Purposelentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo
Plasmid#128455Purposelentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_8.4_Ex_CAMKIIA-EGFP-V5-SV40-Zeo
Plasmid#128464Purposelentiviral expression vector with CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertCAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-Mycn
Plasmid#60667PurposePiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoterDepositorInsertMycn (Mycn Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA822G, A823C, A824TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E 5X C120 c-fos min pro (JDW 1241)
Plasmid#224544PurposeA Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoterDepositorInsert5x-C120-c-Fos promoter
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188747PurposeDonor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188743PurposeDonor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterRSVAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188744PurposeDonor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188745PurposeDonor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchr21Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only