We narrowed to 687 results for: emd

1. pTMS1

   Plasmid

   #87799

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS2

   Plasmid

   #87798

   Purpose

   For constructing SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTH848-gst-disec

   Plasmid

   #89589

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-dis-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-eIF4E

   Plasmid

   #37228

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   eIF4E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET-TBZ

   Plasmid

   #28239

   Depositor

   Yawen Bai

   Article

   Zhou et al Nat Struct Mol Biol. 2008 Aug . 15(8):86

   Insert

   single chain Truncated H2B-H2A.Z
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pTMS2

   Plasmid

   #87800

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTNS1

   Plasmid

   #87801

   Purpose

   For constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7-AglC-His6

   Plasmid

   #89726

   Purpose

   Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   AglC
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7-WbpB-His6

   Plasmid

   #89711

   Purpose

   Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   WbpB (wbpB Pseudomonas aeruginosa)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T7-WbpE-His6

    Plasmid

    #89712

    Purpose

    Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    WbpE (wbpE Pseudomonas aeruginosa)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. T7-WbpD-His6

    Plasmid

    #89713

    Purpose

    Expresses WbpD from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    WbpD (wbpD Pseudomonas aeruginosa)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T7-AglK-His

    Plasmid

    #89714

    Purpose

    Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglK
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3243 pSHLox Hox 11 L2

    Plasmid

    #8762

    Depositor

    Stanley Korsmeyer

    Article

    Shirasawa et al Nat Med 1997 Jun;3(6):646-50.

    Insert

    Hox11 L2 (Tlx3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. Axin GID 1-2/pET29b

    Plasmid

    #16307

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

    Insert

    Axin GID 1-2 (axin1.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acids 320-429

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

15. p-preSufI(IAC)

    Plasmid

    #168516

    Purpose

    Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    pre-SufI(IAC)
    

    Use

    Tags

    6xHisC tag and HSV tag

    Expression

    Bacterial

    Mutation

    C17I and C295A

    Promoter

    T7

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30-T7-lacO-sfGFP

    Plasmid

    #180756

    Purpose

    pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-11-LECT2-V40

    Plasmid

    #183031

    Purpose

    Expression of LECT2 V40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V40 variant

    Promoter

    T7

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

    Plasmid

    #170767

    Purpose

    Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Inserts

    M.XbaI
    M.EcoRI
    M.SalI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

    Plasmid

    #170768

    Purpose

    Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Inserts

    M.XhoI
    M.MunI
    M.Ocy1ORF8430P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Prp39-pET21b

    Plasmid

    #111286

    Purpose

    Prp39 expression plasmid

    Depositor

    Christine Guthrie

    Article

    Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

    Insert

    PRP39
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge