Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 610 results for: prime editing

Clear keywords
Showing: 121 - 140 of 610 results

  1. PEA1-Nuclease-GFP

    Plasmid
    #171994
    Purpose
    Delivers all prime editing nuclease components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171994-plasmid-map-sequence-id-338231

    Enlarge

  2. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206274-plasmid-map-sequence-id-406650

    Enlarge

  7. iPEmaxΔR-D10A

    Plasmid
    #239381
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K and M-MLV-RTΔRNase H in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9(D10A-R221K-N394K), M-MLV-RT(ΔRNase H)
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9; ΔRNase H in M-MLV RT
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239381-plasmid-map-sequence-id-481782

    Enlarge

  17. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

    Enlarge

  18. pU6:2-U6:3-tevopreq1

    Plasmid
    #190910
    Purpose
    Expression of nicking sgRNA and epegRNA under control of separate Drosophila U6:2 and U6:3 promoters. Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Insert
    Empty Backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190910-plasmid-map-sequence-id-373034

    Enlarge

  19. pPPEM

    Plasmid
    #162467
    Purpose
    Destination Expression vector for monocot plant primer editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Insert
    Cas9H840A
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    maize Ubi
    Available Since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    162467-plasmid-map-sequence-id-350385

    Enlarge

Showing: 121 - 140 of 610 results