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Showing: 2071 - 2100 of 20869 results

  1. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid
    #221160
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221160-plasmid-map-sequence-id-450956

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  4. pLSLR

    Plasmid
    #51500
    Purpose
    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51500-plasmid-map-sequence-id-78614

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  5. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

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  7. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  11. pCR3.1-mutLuc

    Plasmid
    #106458
    Purpose
    luciferase reporter, CMV promoter (3 NFkB sites mutated)
    Depositor
    Georg Weber
    Article
    He et al Biochem Biophys Res Commun. 2004 Aug 13;
    Insert
    luciferase reporter, CMV promoter (3 NFkB sites mutated)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    3 NFkB sites mutated
    Promoter
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    106458-plasmid-map-sequence-id-207856

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  12. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

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  13. p35ST

    Plasmid
    #51514
    Purpose
    pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    T30 TALEN pair
    us:NPTII repair template
    Use
    Plant expression t-dna
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51514-plasmid-map-sequence-id-78686

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  16. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Chris MacDonald, Mark Leake
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Use
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

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  22. pRVV700

    Plasmid
    #119017
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    119017-plasmid-map-sequence-id-232904

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  23. pRVV609

    Plasmid
    #119014
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119014-plasmid-map-sequence-id-232795

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  24. pRVV688

    Plasmid
    #119015
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119015-plasmid-map-sequence-id-232899

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  25. pRVV689

    Plasmid
    #119016
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119016-plasmid-map-sequence-id-232901

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Showing: 2071 - 2100 of 20869 results