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pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pFA6a-link-GFPγ-SpHis5

Plasmid

#60781

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPγ

Use

Yeast integrating

Mutation

T65C S72A V163A I167T L231H

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Keratin 8 S431A

Plasmid

#18058

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S431A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3_AKAR5Δ

Plasmid

#119912

Purpose

Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

AKAR5Δ

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I N90

Plasmid

#22186

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Tags

GST

Expression

Mammalian

Mutation

Contains the first 90 aa of TFII-I

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARγ

Plasmid

#119915

Purpose

Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARγ

Expression

Mammalian

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Tags

EGFP

Expression

Mammalian

Promoter

hsp70

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 DN HA

Plasmid

#27651

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

D146N (Dominant Negative)

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
Keratin 8 G61C

Plasmid

#18055

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

G61C

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Keratin 8 S23A

Plasmid

#18057

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S23A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pBS cCollagen XII (CT#1051)

Plasmid

#13916

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Collagen XII (COL12A1 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-C11orf83-V5

Plasmid

#65843

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Tags

V5

Expression

Mammalian

Mutation

WT

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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