Purpose
All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
Inserts
NmeCas9
Nme-sgRNA
Expression
Insect and Mammalian
Mutation
None and fusion of repeat/tracrRNA to make a sgRNA
Promoter
EF-1 alpha and U6
Available Since
Sept. 20, 2018
Availability
Academic Institutions and Nonprofits only