We narrowed to 2,303 results for: dap

1. pCHC004 (∆phaCAB)

   Plasmid

   #226198

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCHC027 (∆pemK)

   Plasmid

   #226204

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Upstream/downstream homology arms for addiction system toxinPemK.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

   Plasmid

   #208869

   Purpose

   For mammalian expression of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1-GST-TEV-GFP-NAP1

   Plasmid

   #208870

   Purpose

   For expression in E. coli of GFP-NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   GFP and GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.HALO-KIF3A tail 419-702

   Plasmid

   #193924

   Purpose

   Mammalian expression of Halo-tagged Kif 3A tail 419-702

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   Kif 3A (KIF3A Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD22Y/PfPRMT5 deletion

   Plasmid

   #203161

   Purpose

   for disruption of PfPRMT5 gene

   Depositor

   Liwang Cui

   Article

   Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

   Insert

   PfPRMT5 fragment
   

   Use

   Tags

   3 myc tags and 3' UTR of P. berghei dihydrof…

   Expression

   Mammalian

   Mutation

   Promoter

   No

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

   Plasmid

   #201533

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   rhaR L128+1bp (frameshift), ilvA G455C

   Promoter

   PrhaBAD (rhamnose)

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

   Plasmid

   #201535

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   rhaS ΔM1-H5, PrhaSR Δ179bp

   Promoter

   PrhaBAD (rhamnose)

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBa.Halo-KIF3B tail 391-747

   Plasmid

   #193929

   Purpose

   Mammalian expression of Halo-tagged Kif 3B tail 391-747

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   Kif 3B (Kif3b Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGST-parallell2-GGA3(571-723)

    Plasmid

    #199150

    Purpose

    Expresses GST-GGA3(571-723) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

    Insert

    GGA3(571-723) (GGA3 Human)
    

    Use

    Tags

    GST and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas4_1_Δ2_VA

    Plasmid

    #186439

    Purpose

    Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCasΔ4_1_2_VA

    Plasmid

    #186440

    Purpose

    Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas-4/1Δ2_VB

    Plasmid

    #186443

    Purpose

    Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas12(PI)

    Plasmid

    #186448

    Purpose

    Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K613A, K617A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas12b

    Plasmid

    #186449

    Purpose

    Encodes Cas12b from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas12b(PI)

    Plasmid

    #186451

    Purpose

    Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R122A, G143P

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACT2-μ1B (137-423)

    Plasmid

    #198172

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1B (137-423)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBT9-AAGAB

    Plasmid

    #198332

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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