We narrowed to 8,596 results for: Set

1. pWPI/hPLK2K111M/Neo

   Plasmid

   #35386

   Depositor

   Anurag Tandon

   Article

   Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

   Insert

   hPLK2 K111M (PLK2 Human)
   

   Use

   Lentiviral

   Tags

   EMCV IRES-Neomycin cassette and V5 epitope and Po…

   Expression

   Mammalian

   Mutation

   K111M

   Promoter

   EF1-alpha

   Available Since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. attB53-Pac-TRE-mCherry-attB53

   Plasmid

   #183611

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   TRE-mCherry
   

   Use

   Rmce

   Promoter

   TRE

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-terminal Halo-XLF

   Plasmid

   #207546

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM55297

   Plasmid

   #153213

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK+ episome

   Plasmid

   #170041

   Purpose

   PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV+ episome

   Plasmid

   #169742

   Purpose

   CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYFP-NEAT1m_v1-RT

   Plasmid

   #97089

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1m_v1-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

   Plasmid

   #91919

   Purpose

   Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Lw2Cas13a and guide expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDragon-empty

    Plasmid

    #155018

    Purpose

    Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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