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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pORTMAGE311B

Plasmid

#120418

Purpose

Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

Depositor

Csaba Pál

Article

Szili et al bioRxiv (2018)

Inserts

MutL E32K
Lambda Beta
XylS

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Pm

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSVL(D3)

Plasmid

#29335

Depositor

John Taylor

Article

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Insert

Trimer of the HDV genome

Expression

Mammalian

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-KIAA1267

Plasmid

#29758

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

KIAA1267 (KANSL1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mWasabi

Plasmid

#169409

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mWasabi

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMo130-TelR

Plasmid

#50799

Purpose

Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

Depositor

Kim Lee Chua

Article

Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

Insert

tellurite-resistance cassette

Use

Suicide vector

Promoter

PCs12

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

Plasmid

#70231

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000563266)

Plasmid

#80036

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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