We narrowed to 8,596 results for: Set

1. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES.Puro.mBeta

   Plasmid

   #24175

   Depositor

   Robert Sobol

   Article

   Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

   Insert

   pIRES.Puro.mBeta (Polb Mouse)
   

   Expression

   Mammalian

   Mutation

   x

   Available Since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDGE671

   Plasmid

   #153237

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQnl_tet

   Plasmid

   #191355

   Purpose

   Clostridium expression vector with TetR-repressed NanoLuc expression

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette
   

   Expression

   Bacterial

   Promoter

   PGus2A-TetO2/1

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC193

   Plasmid

   #118903

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease; mGFP and TwinStr…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-30a(+)-Syk-tSH2

   Plasmid

   #111269

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNAS1b split mCherry 14-3-3β

   Plasmid

   #111880

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Tags

   Split mCherry system: 14-3-3β fused to C-terminal…

   Expression

   Bacterial

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM65893

   Plasmid

   #153216

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-2Aneo

   Plasmid

   #80032

   Purpose

   Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

   Depositor

   Hiroyuki Konishi

   Article

   Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; A platform vector to construct t…

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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