We narrowed to 875 results for: seph

1. pGL3 Pro Mut E2

   Plasmid

   #48750

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutated E-box2 sequence from CACGTT to GCTAGT

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_GWRev_Dest

   Plasmid

   #86954

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

   Depositor

   Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

   Plasmid

   #159660

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

   Plasmid

   #159661

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

   Plasmid

   #159659

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

   Plasmid

   #115137

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2.1 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NG0750 pCI-TPI-PTC48-4H

   Plasmid

   #65803

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Use

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Mutation

   premature stop codon at 48

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD765 FDFT1 3'UTR

   Plasmid

   #105765

   Purpose

   Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

   Depositor

   Joseph Ziegelbauer

   Article

   Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

   Insert

   FDFT1 3'UTR (FDFT1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BRD4 CRISPRi plasmid

    Plasmid

    #154890

    Purpose

    Human BRD4 CRISPRi gRNA

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    BRD4-targeting gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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