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Showing: 2101 - 2130 of 8793 results

  2. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

    Plasmid
    #186045
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8m
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186045-plasmid-map-sequence-id-370173

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  6. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid
    #202036
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato
    Depositor
    Peter Jonas, Yoav Ben-Simon
    Insert
    hM3Dq
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    202036-plasmid-map-sequence-id-405528

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  7. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  8. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

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  9. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  12. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

    Plasmid
    #129111
    Purpose
    Homologous recombination vector for excision reporter 1 in the CLYBL locus.
    Depositor
    Bruce Conklin
    Insert
    LSL, eGFP, NLS-RFP
    Use
    Cre/Lox and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    129111-plasmid-map-sequence-id-252853

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  15. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid
    #202037
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato
    Depositor
    Peter Jonas, Yoav Ben-Simon
    Insert
    hM4Di
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    202037-plasmid-map-sequence-id-405580

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  20. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

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  21. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40131-plasmid-map-sequence-id-51019

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  22. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Article
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Insert
    DD-dCas9VP192-T2A-EGFP
    Use
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69534-plasmid-map-sequence-id-122831

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  26. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104496
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104496-plasmid-map-sequence-id-474734

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Showing: 2101 - 2130 of 8793 results