Addgene

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pEPYC0CM0522

Plasmid

#196158

Purpose

Phytobricks Level 0 part: MinSyn_305

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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bpNLS-DddIA

Plasmid

#187847

Purpose

expresses the bpNLS-linked DddIA in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

bpNLS-DddIA-bpNLS-p2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FBXO7_Halo_C_allele

Plasmid

#178158

Purpose

Donor vector for endogenous tagging of human FBXO7 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FBXO7 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL1269R

Plasmid

#118994

Purpose

Marker fusion of Bar:mCherry (without start codon)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

Bar:mCherry

Use

Fungal expression

Tags

Expression

Mutation

Promoter

promoter less

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1259

Plasmid

#118996

Purpose

GFP (without start and stop codons) in pFGL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression

Tags

Expression

Mutation

Promoter

promoter less

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK594

Plasmid

#71588

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-T Dmel Or49a

Plasmid

#59661

Purpose

Probe for Drosophila melanogaster Or49a expression

Depositor

Leslie Vosshall, Richard Axel

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or49a (Or49a Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(G217S/T499I)

Plasmid

#1158

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

GAL1:10 promoter

Expression

Yeast

Mutation

G217S/T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1

Plasmid

#124650

Purpose

Soma-localized C1V1(t/t) for dense expression in excitatory neurons

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Has Service

AAV9

Insert

C1V1

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-IVT

Plasmid

#215834

Purpose

Template for in vitro transcription of CBE6b (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6b

Plasmid

#215830

Purpose

Express CBE6b (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-eNme2C-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCambia-PUP-IT

Plasmid

#186478

Purpose

Expresses POI-PUP-IT fusion in Plants

Depositor

Jen Sheen

Article

Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

Insert

FLAG-Pup(E)

Use

Tags

Triple Flag Tag

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6c

Plasmid

#215831

Purpose

Express CBE6c (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-eNme2C-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a-IVT

Plasmid

#215833

Purpose

Template for in vitro transcription of CBE6a (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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