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pAc5.1B-EGFP-DmNanos_AC

Plasmid

#148455

Purpose

Insect Expression of DmNanos

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos (nos Fly)

Use

Tags

Expression

Insect

Mutation

sequence contains a deletion of N283 and a mutati…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNanos_1-296_AC

Plasmid

#148456

Purpose

Insect Expression of DmNanos_1-296

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_1-296 (nos Fly)

Use

Tags

Expression

Insect

Mutation

sequence contains a deletion of N283 compared to …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNanos_AC

Plasmid

#148460

Purpose

Insect Expression of DmNanos

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos (nos Fly)

Use

Tags

Expression

Insect

Mutation

sequence contains a deletion of N283 compared to …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNanos_1-296_AC

Plasmid

#148461

Purpose

Insect Expression of DmNanos_1-296

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_1-296 (nos Fly)

Use

Tags

Expression

Insect

Mutation

sequence contains a deletion of N283 compared to …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNanos_del 50-236_AC

Plasmid

#148462

Purpose

Insect Expression of DmNanos-del50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-del50-236 (nos Fly)

Use

Tags

Expression

Insect

Mutation

sequence contains a deletion of N283 compared to …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-GST-DmNanos_50-236_AC

Plasmid

#148465

Purpose

Insect Expression of DmNanos_50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_50-236 (nos Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation D121G compared to the seq…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_AC

Plasmid

#148467

Purpose

Insect Expression of DmNanos_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-F152E (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_AC

Plasmid

#148468

Purpose

Insect Expression of DmNanos_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-L127DF130D (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_50-236-del116-163-GB1opt_AB

Plasmid

#148330

Purpose

Bacterial Expression of DmNanos-opt_50-236-del116-163

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_50-236-del116-163 (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-L127DF130D_AB

Plasmid

#148333

Purpose

Bacterial Expression of DmNanos-opt_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-L127DF130D (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-H1949D_AB

Plasmid

#148335

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-H1949D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot1iso1_1833-2361-H1949D (CNOT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-V1880E_AB

Plasmid

#148336

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-V1880E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot1iso1_1833-2361-V1880E (CNOT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_578-634-GB1_AG

Plasmid

#148744

Purpose

Bacterial Expression of DmeIF4G_578-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-634 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-1

Plasmid

#184834

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-NT

Plasmid

#184835

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-MS2-HA-C1-HsGIGYF1_1-671_AI

Plasmid

#148958

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsGIGYF1_AI

Plasmid

#148961

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF1_1-671_AI

Plasmid

#148943

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF1-F306AF312A_AI

Plasmid

#148945

Purpose

Mammalian Expression of HsGIGYF1-F306AF312A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1-F306AF312A (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge