We narrowed to 2,707 results for: EXO

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pET28a-PKM2-H391Y

Plasmid

#122692

Purpose

Express His-tagged PKM2 (H391Y) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

H391Y

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW110032

Plasmid

#222438

Purpose

Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00011

Plasmid

#222439

Purpose

Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS3

Plasmid

#128107

Purpose

Express HA tagged Nrxn1α-LNS3

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS3 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS1

Plasmid

#128105

Purpose

Express HA tagged Nrxn1α-LNS1

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS1 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCHW110031

Plasmid

#222437

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FMRP shRNA

Plasmid

#206074

Purpose

expression of FMRP RNA for gene silencing in mammalian cells

Depositor

Annette Dolphin

Article

Ferron et al Nat Commun. 2014 Apr 7;5:3628. doi: 10.1

Insert

FMRP (Fmr1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLCHKO

Plasmid

#155048

Purpose

Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCHW100002

Plasmid

#222440

Purpose

Level 0, CDS module (AATG-GCTT) containing rice OsL5.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

OsL5 (Oryza sativa)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9B-HA

Plasmid

#209231

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26567

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons a10, 31a, 37b, and 46. Deletion…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pX330_Actb sgRNA / hSpCas9

Plasmid

#172832

Purpose

Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

Plasmid

#26568

Depositor

Diane Lipscombe

Article

Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

contains exons a10, 18a, 31a, 37b, and 46. Del…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pX330_ACTB-i4 sgRNA / hSpCas9

Plasmid

#172828

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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