We narrowed to 1,873 results for: FRT

1. FFiBlast p5

   Plasmid

   #68470

   Purpose

   Inverted and floxed blasticidin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKF-P14MM2AG5h

   Plasmid

   #128254

   Purpose

   Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-puro-eGFP-HEC1-9A

   Plasmid

   #114023

   Purpose

   Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 9A

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDREV-0

   Plasmid

   #26750

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   H2B-Venus CDS (H2BC3 Human)
   

   Use

   Cre/Lox; Flp/frt; dre/rox

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-hygro-eGFP-HEC1-9D

   Plasmid

   #114029

   Purpose

   Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 9D

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDREV-1

   Plasmid

   #26751

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   H2B-Venus CDS (H2BC3 Human)
   

   Use

   Cre/Lox; Flp/frt; dre/rox

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-hygro-eGFP-HEC1-8A/S55D

   Plasmid

   #114032

   Purpose

   Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 8A/S55D

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPYRMandi-ABI

   Plasmid

   #233649

   Purpose

   Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

   Depositor

   Richard Wombacher

   Article

   Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

   Insert

   TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN-D2irTNG5kwh

   Plasmid

   #128279

   Purpose

   Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   hTetR::NLS::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-D2ir promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-puro-eGFP-HEC1-d80

    Plasmid

    #114025

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-hygro-eGFP-HEC1-d80

    Plasmid

    #114030

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-hygro-eGFP-HEC1-8A/S15D

    Plasmid

    #114031

    Purpose

    Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 8A/S15D

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FR_GFP

    Plasmid

    #31302

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CT691

    Plasmid

    #233660

    Purpose

    BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    BLADE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-tag-N

    Plasmid

    #108078

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-CEP41_Halo

    Plasmid

    #135446

    Purpose

    Mamalian cell expression of HaloTag7 localized at the centrosome

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    CEP41-HaloTag7
    

    Use

    Tags

    CEP41

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. BirA-Myc_N_PAX6

    Plasmid

    #167721

    Purpose

    Expresses PAX6 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    PAX6 (PAX6 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-TOM20_Halo_T2A_EGFP

    Plasmid

    #135443

    Purpose

    Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    TOM20-HaloTag7 and EGFP
    

    Use

    Tags

    TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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