We narrowed to 5,102 results for: codon optimized

1. pLenti-VRER-P2A-Puro

   Plasmid

   #110849

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1-P2A-Puro

   Plasmid

   #110850

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-VQR-PGK-Puro

   Plasmid

   #110855

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available Since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTRdeltaGFPmirGE-DroshaT1.5.5

   Plasmid

   #61084

   Purpose

   pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

   Depositor

   Patrick Salmon

   Article

   Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

   Insert

   deltaGFP
   

   Use

   RNAi

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV302

    Plasmid

    #204973

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    nat1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV299

    Plasmid

    #204967

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3-ARF6-His6(CO)

    Plasmid

    #201887

    Purpose

    Used for bacterial expression of human protein ARF6

    Depositor

    Richard Kahn

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. human Alpha-synuclein

    Plasmid

    #51437

    Purpose

    human codon optimized Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Cloning

    Available Since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pSMH1741

    Plasmid

    #160930

    Purpose

    Expression of soluble Sc octamer (H3)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    HHT1
    

    Tags

    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMH1742

    Plasmid

    #160929

    Purpose

    Expression of soluble Sc octamer (Cse4)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    Cse4
    

    Tags

    6His-TEV, N-terminal 6His-TEV, and none

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid

    #169376

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP1
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid

    #160647

    Purpose

    module containing the human codon optimized Cas9 and the BASTA selection marker

    Depositor

    Diego Orzaez

    Insert

    BASTA / Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, P35S

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103 KIBRA WW domain #1

    Plasmid

    #104229

    Purpose

    Bacterial expression of WW domain #1 from KIBRA

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIBRA WW domain #1 (WWOX Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MYLK4

    Plasmid

    #39175

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MYLK4 (MYLK4 Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Mutation

    N371S, codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only