We narrowed to 13,016 results for: SHI

1. GFP-LiMETER-v1

   Plasmid

   #113933

   Purpose

   Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   LOV2 and Rit polybasic domain (PB)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRFP2-Actin-C-18

   Plasmid

   #178965

   Purpose

   Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   miRFP2-actin
   

   Use

   Tags

   actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AID-C12*-BE4max

   Plasmid

   #216729

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. miCBE

   Plasmid

   #205413

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-tetO-loxP-hNANOG

   Plasmid

   #60849

   Purpose

   Lentiviral DOX-inducible expression vector for human NANOG

   Depositor

   Rudolf Jaenisch

   Article

   Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

   Insert

   hNANOG (NANOG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR - EGFP sgRNA 2

   Plasmid

   #51761

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLXSN(GFP)-myc-BioID-hp100

   Plasmid

   #174738

   Purpose

   Gene overexpression

   Depositor

   Shao-Cong Sun

   Article

   Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

   Insert

   P100 (NFKB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSI-Target-AID-NG

   Plasmid

   #119861

   Purpose

   Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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