We narrowed to 5,819 results for: plasmid dna

1. pEERM1 Km

   Plasmid

   #64024

   Purpose

   Plasmid for integration into psbA2 site of Synechocystis 6803

   Depositor

   Pia Lindberg

   Article

   Englund et al ACS Synth Biol. 2015 Jul 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PpsbA2

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGGY001

   Plasmid

   #48866

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGGY003

   Plasmid

   #48867

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTF-FLPe

   Plasmid

   #139672

   Purpose

   Expression cassette for FLPe recombinase in T-DNA vector

   Depositor

   James Birchler

   Article

   Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

   Insert

   FLPe recombinase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC57-Rluc-BoxB-A114-N40

   Plasmid

   #50562

   Purpose

   Template DNA for in vitro transcription using T7 polymerase

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

   Insert

   Luciferase-BoxB-A114-N40
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PNCA-mCherry

   Plasmid

   #67790

   Purpose

   Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

   Insert

   MMLV-LTR-mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pEERM6 Km

   Plasmid

   #64027

   Purpose

   Plasmid for integration into sqs site of Synechocystis 6803

   Depositor

   Pia Lindberg

   Article

   Englund et al ACS Synth Biol. 2015 Jul 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnrsB

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57-Rluc-let-7-A114-N40

   Plasmid

   #50563

   Purpose

   Template DNA for in vitro transcription using T7 polymerase

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

   Insert

   Luciferase-let7-A114-N40
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR-Blunt_hM3O

   Plasmid

   #112638

   Purpose

   Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

   Depositor

   Ganna Bilousova

   Article

   McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

   Insert

   hM3O
   

   Use

   Pcr template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMVTO-g0

    Plasmid

    #231186

    Purpose

    Non-coding DNA plasmid control

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    g0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTYC10_pPromALB_STARRseq

    Plasmid

    #220289

    Purpose

    STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

    Depositor

    David Waxman

    Article

    Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LT2_33_PyruPro_X2site

    Plasmid

    #194429

    Purpose

    Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    PyruPro
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LT2_34_EthPro_X2site

    Plasmid

    #194430

    Purpose

    Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    EthPro
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTW072

    Plasmid

    #190247

    Purpose

    T-DNA for arabidopsis transformation

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

    Insert

    gRNA, Cas9, firefly luciferase, bar
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTW069

    Plasmid

    #190246

    Purpose

    T-DNA for arabidopsis transformation

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

    Insert

    gRNA, Cas9, firefly luciferase, bar
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR-Blunt_mWasabi

    Plasmid

    #112640

    Purpose

    Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

    Depositor

    Ganna Bilousova

    Article

    McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

    Insert

    mWasabi
    

    Use

    Pcr template

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-Clover

    Plasmid

    #163366

    Purpose

    Backbone for generating N-terminal or C-terminal clover constructs

    Depositor

    Violaine Sée

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag BiBRET Vector

    Plasmid

    #238579

    Purpose

    Express NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. NanoBRET BiBRET Vector, NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag

    Plasmid

    #238572

    Purpose

    Express NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. NanoBRET BiBRET Vector, NanoLuc-KRAS_CRAF(RBD)-HaloTag

    Plasmid

    #238575

    Purpose

    Express NanoLuc-KRAS_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. NanoBRET BiBRET Vector HaloTag-KRAS_BRAF-NanoLuc

    Plasmid

    #238567

    Purpose

    Express HaloTag-KRAS_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. NanoLuc-KRAS(G12V)_CRAF(FL)-HaloTag BiBRET Vector

    Plasmid

    #238571

    Purpose

    Express NanoLuc-KRAS(G12V)_CRAF(FL)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc

    Plasmid

    #238563

    Purpose

    Express HaloTag-KRAS(G12D)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. NanoBRET BiBRET Vector, NanoLuc-KRAS_CRAF(FL)-HaloTag

    Plasmid

    #238564

    Purpose

    Express NanoLuc-KRAS_CRAF(FL)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. NanoBRET BiBRET Vector, HaloTag-KRAS(G12V)_BRAF-NanoLuc

    Plasmid

    #238565

    Purpose

    Express HaloTag-KRAS(G12V)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. NanoBRET BiBRET Vector, HaloTag-KRAS(G12C)_BRAF-NanoLuc

    Plasmid

    #238566

    Purpose

    Express HaloTag-KRAS(G12C)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid

    #237067

    Purpose

    Express LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    LgBiT and SmBiT

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid

    #237066

    Purpose

    Express LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    LgBiT and SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. cRAF CRD-RBD: KRAS G12C BiBRET Vector

    Plasmid

    #236872

    Purpose

    Express cRAF CRD-RBD: KRAS G12C in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD)-CRD (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. Nluc-KRAS(G12D)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236867

    Purpose

    Express NanoLuc(R)-KRAS(G12D)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. Nluc-KRAS(G12C)-cRAF(FL)-HaloTag BiBRET Vector

    Plasmid

    #236868

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. Nluc-KRAS(G12C)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236869

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pAc5.1B-EGFP-CG10077-RA_B

    Plasmid

    #145910

    Purpose

    Insect Expression of CG10077-RA

    Depositor

    Elisa Izaurralde

    Insert

    CG10077-RA (CG10077 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    A23T, Q440R, S483L, I492T, inserted G810, H672Y, …

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-HA-CG10077-RB_B

    Plasmid

    #145930

    Purpose

    Insect Expression of CG10077-RB

    Depositor

    Elisa Izaurralde

    Insert

    CG10077-RB (CG10077 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent and one non silent mutation H59R com…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. cEF1a-GFP

    Plasmid

    #135671

    Purpose

    Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. cSFFV-GFP

    Plasmid

    #135670

    Purpose

    Introduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiCRISPRv2 neo

    Plasmid

    #98292

    Purpose

    Variant of lentiCRISPRv2 that confers G418 resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQLL-MAG[EF1α-copGFP]

    Plasmid

    #218324

    Purpose

    Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid

    #234952

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234954

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234953

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pQLL-Tc1-1_Xt[EF1α-copGFP]

    Plasmid

    #218325

    Purpose

    Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMV-Tc1-2_ST

    Plasmid

    #217289

    Purpose

    Donor plasmid of Tc1-2_ST Transposon

    Depositor

    Yong E Zhang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of Tc1-2_ST transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMV-piggyBac-1_AMi

    Plasmid

    #217236

    Purpose

    Donor plasmid of piggyBac-1_AMi Transposon

    Depositor

    Yong E Zhang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of piggyBac-1_AMi transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge