We narrowed to 31,550 results for: Eng;

1. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

   Plasmid

   #194751

   Purpose

   To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eNS2B47NS3 S135A

   Plasmid

   #155318

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S135A

   Promoter

   M13

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. eNS2B47NS3 L30SF31S

   Plasmid

   #155319

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L30 to S, F31 to S

   Promoter

   M13

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS-sg:GFP

   Plasmid

   #196296

   Purpose

   35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM-Cas9

   Plasmid

   #196325

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding SpCas9 in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pM-Cas12a

   Plasmid

   #196291

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pS-cr:GFP

   Plasmid

   #196295

   Purpose

   35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
   

   Use

   CRISPR

   Tags

   SpeI-DR-BsaI-DR-SpeI

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Td-CBEmax

   Plasmid

   #196600

   Purpose

   Mammalian expression of Td-CBEmax

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF.GFP

    Plasmid

    #17616

    Depositor

    Linzhao Cheng

    Article

    Cui et al Blood. 2002 Jan 15. 99(2):399-408.

    Insert

    EF-1a promoter (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eTd-CBE

    Plasmid

    #196601

    Purpose

    Mammalian expression of eTd-CBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pW34

    Plasmid

    #158210

    Purpose

    LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    LuxABCDE
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK Cas9-2A-Citrine

    Plasmid

    #92393

    Purpose

    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. eTd-CBEm

    Plasmid

    #196602

    Purpose

    Mammalian expression of eTd-CBEm

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pW5Y_Apr

    Plasmid

    #158211

    Purpose

    BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gNS2B47NS3 L30SF31S

    Plasmid

    #155316

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

    Plasmid

    #194755

    Purpose

    Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

    Plasmid

    #194753

    Purpose

    Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge