We narrowed to 6,001 results for: crispr cas9 expression plasmids

1. pCB-CBEv2

   Plasmid

   #221139

   Purpose

   Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptac

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB-CBEv1

   Plasmid

   #221138

   Purpose

   Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptac

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP568-3

   Plasmid

   #70048

   Purpose

   co-expression of Cas9 and crRNA 589 target sequence

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   sgRNA for dpy‐10
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AP568-2

   Plasmid

   #70047

   Purpose

   co-expression of Cas9 and crRNA 589 target sequence

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   sgRNA for dpy‐10
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT11

   Plasmid

   #204290

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAT75

   Plasmid

   #204306

   Purpose

   Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT62

   Plasmid

   #204301

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT43

   Plasmid

   #204299

   Purpose

   For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT56

   Plasmid

   #204300

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAS1yl

    Plasmid

    #73226

    Purpose

    Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; In y. lipolytica

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP025

    Plasmid

    #103874

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP013

    Plasmid

    #103873

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SCP1-dSa-VPR mini.

    Plasmid

    #99685

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-dSa-VPR mini.

    Plasmid

    #99686

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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